Adding properties to request object

Hello, when I add the property to the HTTP request object for how long do I have access to it? Does it expire after user makes a request to the another route?
For example:

app.get('/', (req, res, next) => {
   req.propertyOne = 1;
}, (req, res) => {

When this propertyOne from the example above is deleted? If the user would make a request to the another route then would it be another instace of the request object so it would not contain the propertyOne?